Journal: Frontiers in Pharmacology
Article Title: Integrated network pharmacology and experimental verification to reveal the mechanisms of curcumin in the treatment of colorectal cancer
doi: 10.3389/fphar.2025.1703562
Figure Lengend Snippet: Curcumin promotes ferroptosis and regulates Wnt/β-catenin signaling pathway in vitro . (A) Representative images of transmission electron microscopy observation of mitochondria; curcumin induced reduced mitochondrial volume and increased membrane density in HCT116 cell lines (scale bar: 2 μm and 500 nm); (B) Relative mitochondrial area in HCT116 cell lines. (C) Relative mitochondrial density in HCT116 cell lines. (D–E) Curcumin induced the accumulation of Lipid ROS in CRC cells. Dose-dependent accumulation of Lipid ROS in HCT116 and DLD1 cells treated with curcumin (5–20 μm). (F–G) Curcumin effectively inhibited the expression of SLC7A11 and GPX4, downregulated level of β-catenin and promoted phosphorylation of GSK3β at Ser9. *P < 0.05, **P < 0.01 and ***P < 0.001.
Article Snippet: The human CRC cell line DLD1 (ATCC, United States) was cultured as described and seeded in 96-well plates to reach 80% confluence before treatment.
Techniques: In Vitro, Transmission Assay, Electron Microscopy, Membrane, Expressing, Phospho-proteomics